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. 2015 Jan 7;290(9):5341–5353. doi: 10.1074/jbc.M114.626556

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Precise localization of N and C termini of DRC3 and the C terminus of DRC4 in the N-DRC of Chlamydomonas axonemes. A modified three-dimensional isosurface image shows the locations of the SNAP-biotin-strep-Au labels (gold) at the N and C termini of DRC3 and the C terminus of DRC4 in Chlamydomonas axoneme. Note that the densities of the predicted locations of DRC3 and DRC4 are colored in green and magenta, respectively. The image was generated by merging the information from the averages of labeled SNAP-DRC3, DRC3-SNAP, and DRC4-SNAP axonemes. The modified image shows that the N terminus of DRC3 is close to the basal portion of the linker and to DRC4, whereas the C terminus of DRC3 (green) is close to the central portion of the linker and located at the edge of the L1 projection that is connected to dynein g. The DRC4 extends along the entire base plate of the N-DRC (magenta) with its C terminus located close to the hole in the A-B inner junction. The N terminus of DRC4 has not been localized; therefore, it cannot be excluded that DRC4 also locates along the nexin linker.