TABLE 2.
Image processing information for strains used in this study
| Name | Strain | Tomograms included | Averaged repeats | Resolutiona |
|
|---|---|---|---|---|---|
| DMT | DRC | ||||
| nm | |||||
| WTb | CC-125, 137c mt+ | 9 | 1225 | 3.1 | 3.4 |
| pf2 | CC-4483, pf2-4 | 7 | 983 | 3.8 | 4.2 |
| DRC4-SNAP | pf2-4::DRC4-SNAP | 3 | 459 | 4.5 | 4.8 |
| Labeled DRC4-SNAP | pf2-4::DRC4-SNAP | 10 | 1514 | 3.0 | 3.5 |
| drc3c | drc3 | 6 | 940 | 3.2 | 3.9 |
| DRC3-SNAPc | drc3::DRC3-SNAP | 8 | 1180 | 3.3 | 3.8 |
| Labeled DRC3-SNAP | drc3::DRC3-SNAP | 7 | 1212 | 3.4 | 3.9 |
| SNAP-DRC3 | drc3::SNAP-DRC3 | 6 | 993 | 3.4 | 3.9 |
| Labeled SNAP-DRC3 | drc3::SNAP-DRC3 | 9 | 1479 | 3.5 | 3.9 |
a The 0.5 Fourier shell correlation criterion was used to estimate the resolution at two different locations as follows: the doublet microtubule (DMT; which usually has the highest resolution in the axonemal averages) and the N-DRC (which is usually at lower resolution, typical for associated complexes).
b WT was used as the control and its structure was refined using data originally published by Awata et al. (see Footnote 4).
c The drc3 and DRC3-SNAP structures were refined using data originally published by Awata et al. (see Footnote 4).