TABLE 2.

Comparison of the acceptor specificity of ChGn-1 or co-transfection of ChGn-1 and XYLP secreted into culture medium by transfected COS-1 cells

Acceptor substrate	GalNAc-transferase activitya
	ChGn-1	ChGn-1/XYLP
	pmol/mg/h
GlcUA-Gal-Gal-Xyl-thrombomodulin	0.05 ± 0.01	0.06 ± 0.01
GlcUA-Gal-Gal-Xyl(2P)-thrombomodulinb	1.34 ± 0.8	1.8 ± 0.2
GlcUA-Gal-Gal-Xyl-O-Ser-Gly-Trp-Pro-Asp-Gly	NDc	ND
GlcUA-Gal-Gal-Xyl(2P)-O-Ser-Gly-Trp-Pro-Asp-Gly	ND	62.6 ± 5.7

^a The values are the mean ± S.D. of three measurements.

^b 2P represents 2-O-phosphate.

^c ND, not detected (<0.01 pmol/mg/h).