Subunits of Nox2 complex are present in HPMECs.
A, Nox2 subunits were quantified in control and LPS-treated (15 min) HPMEC lysates by immunoblotting (n = 3). B and C, gp91phox was immunoprecipitated from control and LPS-treated (15 and 30 min) HPMECs, followed by immunoblotting for p47phox (B) with densitometry for the blots shown (C). *, p = 0.009 (control versus 15 min LPS); **, p < 0.01 (control versus 30 min LPS) (n = 3).