Skip to main content
. 2015 Jan 2;290(9):5543–5555. doi: 10.1074/jbc.M114.619817

FIGURE 1.

FIGURE 1.

A β2GPI-reactive T cell hybridoma from C57BL/6 (H-2b) mice recognizes peptide 23 in Domain II of β2GPI. A, β2GPI recombinant fragments consisting of different combinations of domains were used to evaluate T cell epitope specificity. The recombinant fragments (shown schematically) are GP-1 (Domains I and II), GP-2 (Domains III and IV), GP-3 (Domains IV and V), and GP-F (full-length β2GPI). B, the C3hB-1.5 β2GPI-specific T cell hybridoma was evaluated for recognition of recombinant fragments of human β2GPI and twenty-six 15-mer peptides with a 10-residue overlap and encompassing Domains I and II of β2GPI. Human β2GPI (20 μg/ml), recombinant fragments (20 μg/ml), or peptides (10 μg/ml) were incubated with C3hB-1.5 cells and C57BL/6-derived APCs for 24 h. The IL-2 concentration in the supernatant was measured by ELISA. Values represent the mean IL-2 concentration (ng/ml) ± S.E. of triplicate samples, and the data shown are representative of three independent experiments.