FIGURE 2.

Domain and epitope specificity of the β₂GPI-reactive T cell response varies with MHC class II haplotype. Splenic T cells from β₂GPI/LPS-immunized mice (C57BL/6 (H-2^b) (A), BALB/c (H-2^d) (B), or C3H/HeN (H-2^k) (B)) were plated with strain-matched APCs. β₂GPI, GP-1 (Domains I and II), GP-2 (Domains III and IV), GP-3 (Domains IV and V), GP-F (full-length β₂GPI), or MalBP (control fusion protein) was added to the culture at a concentration of 20 μg/ml, and individual peptides from Domains I and II were added at 10 μg/ml. Cells were incubated for 48 h, and IFN-γ production in the supernatant was measured by ELISA. Values represent the mean IFN-γ concentration (pg/ml) ± S.E. of duplicate samples, and the data shown are pooled from three independent experiments.