FIGURE 3.

β₂GPI T cell epitope specificity segregates with MHC class II haplotype. Splenic T cells from B6.C (C57BL/6 with H-2^d) or C.B10 (BALB/c with H-2^b) mice (A) or 129S1 (H-2^b) mice (B) that had been immunized with β₂GPI and LPS were plated with MHC class II haplotype-matched APCs. β₂GPI, GP-1 (Domains I and II), GP-2 (Domains III and IV), GP-3 (Domains IV and V), GP-F (full-length β₂GPI), or MalBP (control fusion protein) was added to the culture at concentration of 20 μg/ml, and individual peptides from Domains I and II were added at 10 μg/ml. Cells were incubated for 48 h, and IFN-γ production in the supernatant was measured by ELISA. Values represent the mean IFN-γ concentration (pg/ml) ± S.E. of duplicate samples, and the data shown are representative of three independent experiments.