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. 2004 Jul;24(14):6231–6240. doi: 10.1128/MCB.24.14.6231-6240.2004

FIG. 1.

FIG. 1.

Transgene expression in S6K1 and S6K2 transgenic mice. (A) Cardiac tissue lysates from NTg and S6K1 transgenic mice (WT, WTS6K1; RR, RRS6K1; KD, KDS6K1) were probed with an anti-HA epitope tag antibody (top panel), an anti-S6K1 antibody (middle panel), and an anti-GAPDH antibody (bottom panel). Two bands were present when probing with S6K1 because a start codon is present before the HA tag, in addition to one at the start of the S6K1 gene. (B) Cardiac tissue lysates from NTg and S6K2 transgenic mice (WT, WTS6K2; KD, KDS6K2) were probed with an anti-HA epitope tag antibody (top panel), an anti-S6K2 antibody (middle panel), and an anti-GAPDH antibody (bottom panel).