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Intravenous bolus injection of IGF1 in S6K+/+, S6K1−/−, S6K2−/−, and S6K1−/− S6K2−/− mice. Hearts were collected 15 min after the injection. S6K1 activity (upper panel), immunoprecipitated S6K1 antibody in heart lysates; S6K1 protein (middle panel), immunoprecipitated S6K1 antibody; S6 phosphorylation (lower panel), direct Western blotting. N, no injection; PBS, PBS injection.
