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. 2015 Jan 13;290(9):5725–5738. doi: 10.1074/jbc.M114.635474

FIGURE 5.

FIGURE 5.

Silencing of UBC9 affects interactions between calnexin and PTP1B. A, NIH3T3 cells were treated with scrambled siRNA (sc siRNA) or UBC9 specific siRNA. Cells were also transfected with YN-CNX and YC-PTP1B expression vectors for BiFC analysis. Western blot analysis was carried out with anti-GFP, anti-UBC9, and anti-β-tubulin antibodies. β-tubulin was used as a loading control. * indicates the location of nonspecific protein bands. The location of the YN-calnexin and YC-PTP1B is indicated by the arrows. The data represent more than three biological replicates. B, BiFC in NIH3T3 cells expressing YN-calnexin and YC-PTP1B fusion proteins with silenced UBC9. sc siRNA, scrambled small interference RNA; siRNA, small interference RNA; The data represent more than three biological replicates. C, quantitative analysis of BiFC (YFP) signals. The data represent more than three biological replicates. *, p < 0.05.