FIGURE 2.
DCC immunoprecipitates contain PKA activity. A and B, E18 rat brains were homogenized in modified RIPA buffer and immunoprecipitated (IP) with antibodies against AKAP79 or DCC or with non-immune IgG as indicated. Immunoprecipitates were incubated with PKA substrate (GFP-R4S) in the absence or presence of cAMP and PKI peptide as indicated. Reaction products were separated by SDS-PAGE and analyzed by immunoblotting with the indicated antibodies to assess immunoprecipitation, substrate levels, and substrate phosphorylation.