FIGURE 2.
Serum anti-SRBC IgG titers following SRBC immunization decline with age in WT but not p27T187A/T187A mice. A, unimmunized WT or p27T187A/T187A, young or older mice show similar base level serum anti-SRBC IgG titer. B, serum anti-SRBC IgG was measured 10 days after SRBC immunization. WT represents p27+/+, T187A for p27T187A/T187A. young mice were between 10 and 12 weeks old; older mice between 15 and 20 months old. Each data point represents one individual, and column heights show medians. Statistical analysis by two-way ANOVA with these four groups of data showed p = 0.0129 for differences between young and older, and p = 0.0036 for differences between WT and T187A. Unpaired two-tailed Student's t tests were used to determine p values between various pairs of samples as marked in the plot.