FIGURE 8.
Affinity of c-di-AMP for RSH. Whole cell lysates of E. coli cells containing an empty vector (EV) or expressing either His-tagged KtrA or His-RSH (A) and purified MBP, MBP-KtrA, and MBP-RSH proteins (40 μm) (B) were mixed with 32P-labeled c-di-AMP in the presence of an excess of cold ATP, to ensure specificity, and c-di-AMP binding was examined. Fraction bound values were determined as described previously (39). Binding assays were performed twice. Averages and standard deviations are plotted.