TABLE 2.
Primers used in this study
Restriction sites in primer sequences are underlined.
| No. | Name | Sequence |
|---|---|---|
| ANG928 | F-KpnI-dacA | TTTGGTACCTATTACCCGGAGGAGATG |
| ANG929 | R-EcoRI-dacA | CCCGAATTCCATATTATTTCACACCTT |
| ANG946 | F-AttB1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTTC |
| ANG947 | R-AttB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTC |
| ANG1191 | R3′up-5′Kan-dacA | CATTTTAGCCATAACATCTCCTCCGGGTAATATTTT |
| ANG1192 | F5′Kan-3′up-dacA | GGAGGAGATGTTATGGCTAAAATGAGAATATCACCG |
| ANG1193 | R3′Kan-5′down-dacA | CAAGCAACTCTTCTAAAACAATTCATCCAGTAAAAT |
| ANG1194 | F5′down-3′Kan-dacA | GAATTGTTTTAGAAGAGTTGCTTGCTGAACATTGGT |
| ANG1195 | R-attB2-dacA | ACAAGAAAGCTGGGTCCATTTATATAAGCCTTCGTTTCACTTGGTTG |
| ANG1196 | F-dacA-check | GTGTAGCTATTGCTTGATAATGG |
| ANG1197 | R-dacA-check | CCTAATTTAAATGCCAATTCAGGTG |
| ANG1218 | F-attB1-dacA | ACAAAAAAGCAGGCTTCGAGGTTTCAGCATCAATTGAAAATAACAG |
| ANG1450 | F-AvrII-dacA | GGGCCTAGGTATTACCCGGAGGAGATG |
| ANG1451 | R-SacII-dacA | GGGCCGCGGCATATTATTTCACACCTT |
| ANG1704 | F-NdeI-relA | GGGCATATGGTTGCGGTAAGAAGTGCACATATC |
| ANG1705 | R-EcoRI-relA | AAAGAATTCCTAACTCCCGTGCAACCGACGC |
| ANG1707 | F-attB-RSH | ACAAAAAAGCAGGCTTCATTAGTTGAAAAATTAGGCGGTATCGTAGT |
| ANG1708 | R-attB-RSH | ACAAGAAAGCTGGGTCCAAATCCTACAGCTGCGAATAAATCATCTT |
| ANG1709 | R-YQS-RSH | TGTAGTATGCAACAAATTTTGTTTAGGCATTGCAAT |
| ANG1710 | F-YQS-RSH | AAACAAAATTTGTTGCATACTACAGTAGTAGGCCCA |
| ANG1721 | F-NcoI-gppA | GGGCCATGGGTTCCACCTCGTCGCTG |
| ANG1722 | R-HindIII-gppA | GGGAAGCTTATGCACTTCCAGCGGCCAG |
| ANG1724 | F-RSH-check | CGCAAAAGACAGAGATGTTG |
| ANG1725 | R-RSH-check | CTAGTTCCAAACTCTTGTTAC |