FIGURE 2.
Primary AEC treated for 2 h with 10 μm DHA and 50 μm CAY10397 resulted in a significant decrease in 14-oxoDHA; *, p ≤ 0. 05 (A). To confirm the identification of 14-oxoDHA, a standard was made from 14-HDoHE oxidation using the Dess Martin periodinane reagent. 14-OxoDHA formed from cell treatment with 2 μm 14-HDoHE (B) and the standard (C) were characterized by retention time and full scan MS2 on a high resolution accurate mass Orbi Velos. FTMS-ESI, Fourier transform mass spectrometry-electrospray ionization.