. 2015 Jan 15;290(9):5912–5925. doi: 10.1074/jbc.M114.606038

TABLE 1.

Kinetic parameters for chimera tRNA transcripts

The relative V_max/K_m for the T. thermophilus wild-type tRNA^Phe transcript was expressed as 100%. ND means that the kinetic parameters could not be calculated correctly.

Transcript name	K_m	V_max	Relative V_max/K_m or relative initial velocity
	nm	μmol mg⁻¹ h⁻¹	%
T. thermophilus
Wild-type tRNA^Phe	130 ± 20	360 ± 60	100

*S. cerevisiae*
Wild-type tRNA^Phe	ND	ND	<5^a
Transcript 1	ND	ND	<5^a
Transcript 2	200 ± 40	450 ± 60	80
Transcript 3	150 ± 40	360 ± 60	70
Transcript 4	100 ± 20	260 ± 50	95
Transcript 5	>500	<130	10
Transcript 6	ND	ND	<5^a
Transcript 7	ND	ND	<5^a
Transcript 8	ND	ND	<5^a
Transcript 9	80 ± 20	200 ± 50	90
Transcript 10	ND	ND	<5^a
Transcript 11	ND	ND	<5^a
Transcript 12	240 ± 50	710 ± 120	130
Transcript 13	180 ± 50	660 ± 100	135
Transcript 14	320 ± 60	450 ± 80	50

^a The relative initial velocities were calculated from the time course experiment for 30 min. <5 means that the relative initial velocity was below 5% of that for T. thermophilus tRNA^Phe.