FIG. 7.

Pitx-1 synergizes with c-Jun/c-Fos in transactivation of the mGnRHR gene promoter. A Luc reporter gene fused to −308/−264-GH₅₀ (A) or −308/C-269T/−264-GH₅₀ (B) was cotransfected into CV-1 cells with either intact or mutant Pitx-1 expression vectors, or with empty vector, pcDNA3. c-Jun and/or c-Fos expression vectors or empty vector, pCMV5, were also cotransfected. Data are plotted in comparison with the empty expression vector control for each reporter. Data are presented as the mean ± SEM of three independent experiments. *, P < 0.05 versus the corresponding group in the absence of Pitx-1 cotransfection; #, P < 0.05 versus intact Pitx-1 alone; **, P < 0.05 versus intact Pitx-1/c-Jun.