FIG. 1.

Construction of chimeric IL-7 receptors and expression on D1 cells. (A) Structures of the chimeric mutant receptors used in this study. Constructs contain the hIL-4R extracellular domain, mIL-7R transmembrane domain (TM), and mIL-7R intracellular region. S, serine-rich domain; T, tyrosine-containing domain. (B) Structure of the pMIG vector used to generate retroviruses. LTR, long terminal repeat; IRES, internal ribosomal entry site; MCS, multiple cloning site. (C) Flow-cytometric analysis of D1 cells infected with the parental retrovirus (Vector) or retroviruses. Cells were sorted for GFP and then stained for surface expression of hIL-4R.