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bcl2 mRNA induction versus mutations and deletions in the intracellular domain of IL-7R. Transfected or nontransfected D1 cells were maintained in mIL-7, washed, and stimulated with either mIL-7 or hIL-4 for 4 h. Total RNA was extracted, and bcl2 transcripts were analyzed by RPA. Induction of bcl2 required both Box1 and Y449. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
