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. 2004 Jul;24(14):6501–6513. doi: 10.1128/MCB.24.14.6501-6513.2004

FIG. 9.

FIG. 9.

Activation of Stat5 in D1 cells following IL-7 stimulation. (A) D1 cells were deprived of IL-7 overnight and then stimulated with mIL-7 for 10 min. (B) D1 cells were deprived of IL-7 overnight and then stimulated with mIL-7 (50 ng/ml) for 2 h. Nuclear extracts were analyzed by EMSA using a labeled Stat5 consensus oligonucleotide. Specificity was evaluated by competition with cold DNA or with an antibody (Ab) against Stat5. Results show that IL-7 induced Stat5 nuclear translocation and DNA binding.