Figure 3.

PBAE delivery of HSVtk plasmid enables GCV-mediated killing of malignant glioma cells in vitro. 9L and F98 cells were transfected with plasmids encoding either GFP or HSVtk and then treated with 0, 5, or 50 μg/mL GCV prodrug. Cells treated with both HSVtk and GCV exhibit 100% cancer cell killing, measured by cell counting, versus GFP-transfected cells treated with GCV, showing that GCV-induced cell killing is dependent on the presence of HSVtk. Additionally, HSVtk without GCV does not kill cells.