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. Author manuscript; available in PMC: 2015 Jul 22.
Published in final edited form as: Nature. 2014 Nov 2;517(7535):501–504. doi: 10.1038/nature13868

Extended Data Figure 4. Delayed antigen elimination does not account for renal disease in GaMD-immunized γ1- mice.

Extended Data Figure 4

a. BALB/c WT and γ1- mice (10/gp) were immunized s.c. withGaMD. Sera obtained 5, 6, 7 and 9 days later were evaluated by gel double diffusion for the presence of goat IgG. b-e. BALB/c WT mice (4 or 5/gp) were injected s.c. with a total of 0.2 ml of different mixtures of GaMD and goat anti-KLH antisera. b, c, Mouse sera collected 9d later were assayed for BUN (b) and IgG1 anti-goat IgG Ab (c). d, Sera obtained 6-13d post-immunization were evaluated by gel double diffusion for the presence of goat IgG. e, Urine samples collected 4-12 days post-immunization were analyzed for protein. * p<0.05, **p<0.005