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. 2014 Dec 23;92(2):56. doi: 10.1095/biolreprod.114.124420

FIG. 7.

FIG. 7

IA IL-1β exposure modestly increased IL-10 mRNA expression and decreased the IL-1RA:IL-1β ratio in chorioamnion-decidua. A) Frequency of CD3+CD4+CD127−CD25+FOXP3+ Tregs in decidua parietalis was measured by flow cytometry. B–D) mRNAs were measured by quantitative RT-PCRs in chorioamnion-decidua and normalized to the expression of eukaryotic 18S rRNA. Values in experimental animals were expressed as fold change relative to a pooled average in the control animals (n = 5). B) IA IL-1β exposure significantly increased IL-10 mRNA but not FOXP3 mRNA. C) A modest increase of IL-1ra mRNA was observed after IA IL-1β 24-h exposure. D) Graph shows a significant decrease of IL-1ra/IL-1β mRNA ratio after IA IL-1β exposure. Graphs show a significant decrease of IL-1ra/IL-1β protein level ratio after IA IL-1β exposure in chorion-amnion decidua (E) and amniotic fluid (F). *P < 0.05 and ‡P = 0.072 compared to controls; #P < 0.05 compared to IA IL-1β 24-h group.