Table 1.
Molecular detection of rickettsiae, anaplasma and ehrlichia and blast analysis of the sequences derived from tick samples in this study
| Tick sample (Species, location) | Rickettsia | Anaplasma 16S rDNA | |||
|---|---|---|---|---|---|
| gltA | ompA | ompB | sca4 | ||
| Candidatus Rickettsia sepangensis | |||||
| S5(A. varanense, Sepang) | R. tamurae strain AT-1 (AF394896) (1033/1043, 99.0%) | R. tamurae strain AT-1 (DQ103259) (417/427, 97.7%) | Unable to be amplified | No significant similarity | A. phagocytophilum (AY551442, 99%, 253/256), A. platys (JX261979, 99% 253/256) |
| Candidatus Rickettsia johorensis | |||||
| P1 (pooled A. helvolum, Johore), S4-2 (A. varanense, Sepang), S6-1 (A. helvolum, Sepang) | R. raoultii strain Khabarovsk (DQ365804) (1057/1060, 99.7%) | R. raoultii strain Khabarovsk (DQ365801) (418/429, 97.4%) | R. raoultii strain Khabarovsk (DQ365798) (762/775, 98.3%) | R. raoultii strain Khabarovsk (DQ365808) (795/816, 97.4%) | Not amplified |
| S7-2 (A. varanense, Sepang) | R. raoultii strain Khabarovsk (DQ365804) (1057/1060, 99.7%) | R. raoultii strain Khabarovsk (DQ365801) (418/429, 97.4%) | R. raoultii strain Khabarovsk (DQ365798) (762/775, 98.3%) | R. raoultii strain Khabarovsk (DQ365808) (795/816, 97.4%) | A. bovis (AB983438, 99%, 253/256) |
| S2, S4 (A. helvolum, Sepang), S6, S7 (A. varanense, Sepang) | Not amplified | A. phagocytophilum (AY551442, 99%, 253/256), A. platys (JX261979, 99%, 253/256) | |||
| S6-2 (A. varanense, Sepang) | Not amplified | A. bovis (AB983438, 99%, 253/256) | |||
| S3, S7-3 (A. varanense, Sepang) | Not amplified | Ehrlichia spp. (J410257, 99%, 249/256) | |||