Table 1.

Sequencing statistics of WES and RNA-seq in SCTG of single MEFs

Sample ID	Number of bases		Overlap with the SureSelect-exome (SSE)*
	sequenced (Gb)	mapped (Gb)	Num. SSE bases covered (bp)	avg. coverage (fold)	Percentage of SSE with coverage of
					≥1X	≥5X	≥10X	≥20X
Bulk1-WES	7.1	7.0	51,779,114	71	99.7	99.6	98.8	95.2
MEF1-WES	5.8	4.7	50,680,965	56	97.6	92	84.9	71.9
MEF2-WES	5.7	5.0	50,907,662	59	98.0	93.1	86.6	74.5
MEF3-WES	6.7	6.1	50,788,767	68	97.8	93.6	87.6	76.5
MEF4-WES	11.3	8.6	47,215,249	61	90.9	85.5	79.6	70.7
Bulk2-WES	7.3	6.3	51,664,417	77	99.5	99.2	97.5	92.4
MEF5-WES	11.1	8.2	49,860,043	101	96.0	91.1	85.6	76.4
MEF6-WES	7.3	6.5	49,012,258	74	94.4	88.9	83.0	73.2
MEF7-WES	12.8	10.0	48,375,846	114	93.1	87.4	82.1	74.0
MEF8-WES	5.3	3.9	46,938,561	48	90.4	78.0	68.4	55.1
MEF9-WES	6.2	4.8	41,899,982	101	81.1	67.7	62.1	55.5
MEF10-WES	5.9	5.1	41,360,563	95	80.1	71.9	66.8	59.5
Bulk-RNAseq	7.9	7.2	28,229,859	34	54.6	42.8	38.3	33.2
MEF1-RNAseq	18.3	6.9	9,778,121	26	18.9	14.2	11.2	7.6
MEF2-RNAseq	16.3	6.7	16,097,775	24	31.2	22.6	16.9	10.8
MEF3-RNAseq	14.8	7.1	14,912,833	13	28.9	16.4	10.0	4.9
MEF4-RNAseq	30.7	13.2	6,912,063	32	13.4	9.3	8.0	6.3
MEF5-RNAseq	15.8	3.7	4,670,310	42	9.0	5.8	5.3	4.6
MEF6-RNAseq	19.3	9.9	8,928,712	35	17.3	12.8	10.9	8.5
MEF7-RNAseq	24.8	10.8	7,162,216	26	13.9	7.7	6.5	5.1
MEF8-RNAseq	16.6	3.7	3,083,821	20	6.0	3.7	2.8	1.8
MEF9-RNAseq	10.9	4.2	1,901,612	31	3.7	2.1	1.9	1.7
MEF10-RNAseq	12.4	4.8	3,059,393	25	5.9	3.4	2.8	2.2

^*The target size of SureSelectExome (SSE): 51,942,733 bp

SCTG was performed in two groups of MEFs. MEF1-4 and MEF8 formed group 1; MEF5-7 and MEF9-10 formed group 2.