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. 2015 Feb 21;14:22. doi: 10.1186/s12934-015-0206-8

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© Li et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of XI and XK in Pichia pastoris . (A) The expression vector containing the synthetic XI gene derived from Orpinomyces spp. (B) The expression vector containing the P. pastoris XK gene. (C) XI and XK expression analysis by SDS-PAGE. Lane 1, GS115 (control); Lane 2, GS-XI; Lane 3, GS-XK; Lane 4, GS-XI-XK; Lane M, protein molecular size marker. The right arrow indicate the position of XI.