Table 2.
The calculated fermentation performance metrics for all engineered Pichia pastoris strains
| Strains | μ (h −1 ) | q s (g/g DCW/h) | Y X/S (g/g DCW) |
|---|---|---|---|
| GS115 | 0.0075 ± 0.0008 | 0.056 ± 0.006 | 0.135 ± 0.004 |
| GS-XI | 0.0107 ± 0.0014 | 0.055 ± 0.007 | 0.194 ± 0.007 |
| GS-XK | 0.0072 ± 0.0007 | 0.059 ± 0.008 | 0.123 ± 0.012 |
| GS-XI-XK | 0.0103 ± 0.0014 | 0.057 ± 0.008 | 0.181 ± 0.010 |
| GS115SB50 | 0.0112 ± 0.0005 | 0.061 ± 0.006 | 0.185 ± 0.015 |
| GS-XISB50 | 0.0193 ± 0.0013 | 0.051 ± 0.004 | 0.378 ± 0.013 |
| GS-XKSB50 | 0.0138 ± 0.0007 | 0.051 ± 0.006 | 0.271 ± 0.027 |
| GS-XI-XKSB50 | 0.0110 ± 0.0013 | 0.092 ± 0.011 | 0.120 ± 0.003 |
μ, specific growth rate; qs, specific xylose consumption rate; YX/S, cell yield on xylose. All metrics were average values calculated based on fermentation data at 72 h of fermentation. The contribution of oligopeptides present in the complex medium to performance metrics was excluded based on data from Figure 1.