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Changes of copy numbers of xylose pathway genes after evolutionary engineering
| Strains | XI | XK | XDH |
|---|---|---|---|
| GS-XISB50/GS-XI | 6.17 ± 0.76 | N.D | N.D |
| GS-XKSB50/GS-XK | — | 0.84 ± 0.27 | N.D |
| GS-XI-XKSB50/GS-XI-XK | 1.27 ± 0.07 | N.D | 1.03 ± 0.19 |
Copy number was determined by real time qPCR. ACT1 gene was utilized as a reference control. Each sample was run in triplicates.
N.D: not determined, because no significant transcriptional changes were observed.
