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. 2015 Feb 27;6:108. doi: 10.3389/fpls.2015.00108

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Copyright © 2015 Wang, Lin, Feng, Chen, Qiu and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcription activation activity of TF TaNAC1. Full coding region (S1), N-terminal part (S2), or C-terminal parts (S3 and S4) of TaNAC1 were fused with the GAL4 DNA-binding domain in vector pGBKT7 (A). Plasmids containing the fusion genes and the empty vector control pGBKT7 were introduced into yeast cells of strain AH109. Yeast transformants of different vectors were plated as shown in the diagram (B) and incubated for 2 days at 28°C on an SD/Trp^- plate (C) and SD/Trp^-/His^-/Ade^- plate (D). α-galactosidase activities were examined by X-α-Gal staining and kept at 28°C for 8 h (E).