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. 1974 Oct;71(10):3892–3895. doi: 10.1073/pnas.71.10.3892

Enzymatic Synthesis of Solid Phase-Bound DNA Sequences Corresponding to Specific Mammalian Genes

Pal Venetianer 1,*, Philip Leder 1
PMCID: PMC434291  PMID: 4139705

Abstract

With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.

Keywords: synthetic DNA, globin mRNA, RNA-dependent DNA polymerase, oligo(dT)-cellulose, chromatography

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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