Figure 5.

Molecular and cellular dynamics during tissue expansion. The tissue that is under the condition marked by the point within the blue area is shown in the phase diagram of figure 4a. (a) The number of cells (solid line) and the density of growth molecules (dotted line) as a function of time. (b) The total amount of growth molecules (solid line) and the total volume of tissue (dotted line) as a function of time. (c) Contributions of degradation (solid line) and dilution (dotted line) to molecular density as a function of time. These contributions are estimated by equation (3.17) using data in (a) and (b). (d) Ratios of cell states as a function of time. Cell states I, II, III, IV and pause are shown by blue, yellow, green, red and grey lines, respectively. (e) Time-series images of the growing epithelial vesicle in the expansion phase. Cells are coloured according to cell state: I (blue), II (yellow), III (green), IV (red) and pause (grey). The process of the cell cycle is also shown in the electronic supplementary material, movie 4. (Online version in colour.)