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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: J Immunol. 2014 Dec 15;194(2):584–594. doi: 10.4049/jimmunol.1401024

Figure 2.

Figure 2

IL-10 activation of the PI3K/Akt pathway requires AMPK. (A and B), IL-10 activates PI3K and Akt in AMPKα1+/+ BMDM. BMDM generated from AMPKα1+/+ and AMPKα1−/− mice were treated with rm-IL-10 (20 ng/ml) for the time points indicated. Total cellular lysates were collected for Western blot assessment of (A) AMPK-Thr172, ACC-Ser79, PI3K-Tyr199, and (B) Akt-Thr308 and Akt-Ser473 phosphorylation. (C), PI3K inhibition does not block IL-10 activation of AMPK. BMDM generated from C57BL/6 mice were incubated with either media alone, or with LY294002 (20 µM) for 1 h, then exposed to rm-IL-10 (20 ng/ml) for the time points indicated. Total cellular lysates were collected for Western blot assessment of Akt-Thr308 and Akt-Ser473 and AMPK-Thr172 phosphorylation. Protein phosphorylation levels were analyzed by densitometry and are displayed as a bar histogram. Results shown are representative of three independent experiments.