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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: J Immunol. 2014 Dec 15;194(2):584–594. doi: 10.4049/jimmunol.1401024

Figure 3.

Figure 3

IL-10-induced AMPK activity promotes mTORC1 activation. BMDM generated from AMPKα1+/+ and AMPKα1−/− mice were treated with rm-IL-10 (20 ng/ml) for the time points indicated. Total cellular lysates were collected for Western blot assessment of (A) mTOR-Ser2448, (B) p70 S6K-Ser371, and (C) TSC2-Ser939 and TSC2-Ser1387 phosphorylation. Protein phosphorylation levels were analyzed by densitometry and are displayed as a bar histogram. Results shown are representative of two to four independent experiments.