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. 2015 Feb 22;14:24. doi: 10.1186/s12934-015-0208-6

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© Qi et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of pH on the solubility of EGFP and MMP-12_CAT inclusion bodies by freeze-thawing method. A constant amount of EGFP and MMP-12_CAT inclusion bodies was solubilized at different pH in 20 mM potassium phosphate buffer containing 2 M urea by freeze-thawing method. (A) SDS-PAGE analysis of effect of pH on the solubility of EGFP inclusion bodies. (B) SDS-PAGE analysis of effect of pH on the solubility of MMP-12_CAT inclusion bodies. (C) Protein bands were quantitated by densitometric analysis using ImageJ Software.