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. 2015 Feb 22;14:24. doi: 10.1186/s12934-015-0208-6

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© Qi et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fluorescence and CD spectra of solubilized EGFP from inclusion bodies. (A) Fluorescence spectra of solubilized EGFP from freeze-thawing method (2 M urea) and traditional urea-denatured method (8 M urea). (B) Far UV CD spectra of solubilized EGFP by freeze-thawing method.