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SDS–PAGE analysis of five solubilized proteins from inclusion bodies. Five inclusion body proteins were suspended in PBS buffer (pH 8) containing 2 M urea and followed by freezing at −20°C and thawing at room temperature. Lane M is marker (170, 130, 100, 70, 55, 40, 35, 25, 15 kDa), T stands for cleaned total inclusion bodies, S stands for solubilized protein supernatants from inclusion bodies.
