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. 2006 Nov 18;82(7):197–207. doi: 10.2183/pjab.82.197

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© 2006 The Japan Academy

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — In vitro induction of heart rudiment in animal caps. (A) The method used to induce cardiac tissues in animal cap cells of Xenopus. Five animal caps were dissociated and treated with 100 ng/mL activin for 5 h, then reaggregated and cultured for 3 days. (B) The transplantation procedure. Explants were implanted as a replacement of the heart rudiment or ectopically. (C) A 10-day-old tad-pole with a well developed ectopic heart that formed after transplantation of activin-treated reaggregates into the abdominal region at the late neurula stage. (arrow; original heart, arrow head; ectopic heart).