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. 2014 Dec 31;15(1):160. doi: 10.1186/s12931-014-0160-8

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© Van de Laar et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Examples of FACS plots for cell surface markers that are heterogeneously expressed on tracheal basal cells. Primary human tracheal basal cells were stained with PE-conjugated antibodies for the indicated markers. FACS staining for strains 23 and 38 were done at the same time using the same control unstained cells and therefore, have the same gates. FACS staining for strain 37 was done at a different time and the gates for this strain are drawn relative to the unstained controls used at that time.