Fig. 5.

The VqmR mRNA targets. (A) Expression of VqmR-controlled target mRNAs was measured in wild-type and ΔvqmR V. cholerae strains. The strains carried either a control plasmid (pctr) or a plasmid harboring the vqmR gene (pvqmR). RNA was monitored using qRT-PCR. Expression in the wild-type strain was set to 1. Error bars represent SD of three replicates. (B) Secondary structure of VqmR. The secondary structure of VqmR was derived from SI Appendix, Fig. S6. Conserved regions R1 and R2 are indicated in red. (C) VqmR regulation of target mRNAs. E. coli harboring plasmids carrying the eight genes denoted on the x axis each fused to gfp were cotransformed with a control plasmid (pctr) or the indicated VqmR-expressing plasmids. gfp and vqmR transcription were driven by constitutive promoters. Strains were grown in LB for 8 h and GFP production was measured. GFP levels in strains carrying the control plasmid (pctr) were set to 1. Error bars represent SD of three replicates.