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. 2015 Feb 2;112(7):E766–E775. doi: 10.1073/pnas.1500203112

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Fig. 6. — Base pairing of VqmR with vc1063 and vpsT. (A) Predicted base pairing between VqmR region R1 and vc1063 mRNA. VqmR region R1 is indicated in blue and the vc1063 Shine Dalgarno sequence is boxed. Mutations introduced in B are indicated with arrows. (B) VqmR repression of vc1063 requires region R1. Vc1063::GFP and Vc1063*::GFP (G-10C) were measured using Western blot. RNAPα served as the loading control. VqmR (PvqmR) and VqmR C63G (PvqmR*) were probed on Northern blot. The 5S rRNA served as loading control. (C) Predicted base pairing between VqmR region R2 and vpsT mRNA. Region R2 is indicated in red and the vpsT Shine Dalgarno sequence is boxed. Mutations introduced in D are indicated with arrows. (D) VqmR repression of vpsT requires region R2. VpsT::GFP and VpsT*::GFP (G-17C) were measured using Western blot. RNAPα served as the loading control. VqmR (PvqmR) and VqmR C94G (PvqmR*) were probed on Northern blot. The 5S rRNA served as loading control.