Fig. 2.

Screens for the presence of the LSR1 and 5AY Buchnera genotypes based on a PCR and diagnostic restriction digest. Each lane is from a screen of a single female aphid; lanes labeled 5AY and LSR1 are controls from stock aphids. LSR1 Buchnera contain a distinctive restriction site, yielding bands of 363 bp and 151 bp for LSR1 and a single band of 514 bp for 5AY. Results for set 1 are shown for the F1, F4, and F11 generations. Some LSR1 Buchnera remained in F4 females but were eliminated by a heat exposure in F5, and this was maintained in future generations, as shown by the assay in F11. In set 2, F1 individuals exhibited complete replacement of the original LSR1 Buchnera by the 5AY Buchnera.