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. 2015 Feb 2;112(7):2121–2126. doi: 10.1073/pnas.1418139112

Fig. 1.

Fig. 1.

ATM down-regulation and inhibition lead to NOX4 up-regulation. (A and B) Elevated levels of NOX4 were found in A-T (A-T: GM05849) compared with control fibroblasts (CTRL: GM00637) (Immunocytochemistry in A, immunoblot in B). (C–E) Inhibition of ATM activity in CTRL cells (CTRL: GM03652 primary fibroblasts) with increasing doses of KU55933 (µM) led to increased levels of NOX4 protein (C, Upper Left and Upper Right, 20× magnification; Lower Left and Lower Right; 40× magnification). (D, Upper) NOX4 detection by immunoblot. (D, Lower) Quantification. (E) NOX4 transcript levels. (F) Down-regulation of ATM in control cells (CTRL: GM03652 primary fibroblasts) with siRNA led to elevated NOX4 levels. *P < 0.05.