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. 2015 Feb 2;112(7):2000–2005. doi: 10.1073/pnas.1416694112

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Fig. 3. — Characterization data of N-linked glycan pools. F8-IL9 samples from TGE (blue), SE (red), TGE after desialylation (DS; gray), and SE after deglycosylation (DG; black) were analyzed. (A) Representative HILIC-HPLC profiles of 2-AB–labeled N-glycan pools after PNGase F release and neuraminidase treatment. (B) Quantification of Neu5Ac by reversed-phase HPLC upon mild acid hydrolysis. (C) Fluorimetric quantification of terminal Neu5Ac after enzymatic release with α2-3,6,8,9 neuraminidase. (D) MALD-TOF/TOF spectra of permethylated glycan pools. All color schemes followed the guidelines of the Consortium for Functional Glycomics (33).