Fig. 1.

Architecture and constructs of the homodimeric TAPL complex. (A) CoreTAPL was modeled on the X-ray structure of Sav1866 (Protein Data Bank ID: 2ONJ) (4). The core complex consists of 2 × 6 transmembrane helices and two nucleotide-binding domains. The accessory N-terminal membrane-embedded domain TMD0 is schematically depicted by four predicted transmembrane helices connected by short loops. (B) A fusion construct of human coreTAPL (637 amino acids, 69.7 kDa) linked to the YFP derivative mVenus via a tobacco etch virus (TEV) cleavage site and a single-cysteine coreTAPL mutant were designed for expression in Pichia pastoris.