Fig. 3.

Packaging signals function cooperatively. (A) CP was titrated into 10 nM Alexa Fluor-488–labeled PS1-5 (black) or B3 (dashed - blue). Details as in legend Fig. 2A. Titration points are color coded for each RNA. (B) CP titration into 10 nM of Alexa Fluor-488–labeled PS1-5 and its sequence variants (X), shown as cartoons (Left). (C) Color-coded size distributions of species present in the titrations shown in A and B (substrate key, Left). Data shown are taken from the titration of 200 nM CP onwards in A and B. For PS1-5 and its variants this is 4,000–6,000 s, and for B3 from 2,000 to 8,000 s. (D) Expanded section of B showing the effects of CP addition on each variant. (E) CP titration into spacing variants (see cartoon; Left) and their size distribution plots (Right). Data shown are from 0 to 5,000 s.