Fig. 3.

Analysis of RNA release in synthetic termination complexes. (A) Outline of experiment. P7, NusA, and antisense oligonucleotides (asDNA) were added to the reconstituted TEC bound to an affinity resin; addition of UTP and ATP resulted in transcription to the end of the U-tract, followed by RNA release into the supernatant fraction. (B) Kinetics of RNA release in TECs stalled at the termination point. The efficiency of RNA release was calculated as a ratio of the amount of released RNA to the sum of the amounts of resin-bound and released RNAs (averages and SDs from three independent experiments are shown). See Fig. S3 for further details.