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Validation of protein phosphorylation changes. (a) SH-SY5Y cells stably expressing either pSuper-GFP vector or pEGFP vector were used as control cells to compare with SH-SY5Y cells expressing pSuper-GFP containing shRNA against PINK1 at two different exonic locations. Western blot analysis of the cell homogenates using antibodies against either non-phosphorylated or phosphorylated GSK3β and c-Jun. Stable cell lines expressing different plasmids are designated.
