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. 2015 Feb 19;15(1):9. doi: 10.1186/s12896-015-0123-2

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© Hauptmann et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Specific immune response of mouse sera determined by indirect ELISA. Samples of 500 ng per well of A) 1xVSO1-100xELP and B) 4x VSO1-100xELP were coated to a microtiter plate and the reaction against mouse sera was analyzed for different dilutions. The antigen-antibody complex was detected using goat anti-mouse IgG conjugated with alkaline phosphatase. Extinction was measured at 405 nm (ordinate).