Table 2.

Elastic penetration moduli of recombinant spidroin-based fusion protein layers

Substance	Elastic penetration modulus E (GPa)
1xVSO1-100xELP	2.73 ± 0.02
2xVSO1-100xELP	3.85 ± 0.02
4xVSO1-100xELP	4.51 ± 0.03
LDPE Riblene®FL30	0.172 ± 0.001

GPa: Gigapascal; ELP: elastin-like peptide.

Values were obtained by AFM-based nanoindentation under the following conditions: temperature = 23°C, relative humidity = 35%, system sensitivity = 22.5 nm/V, spring constant = 59.3 N/m, tip radius = 112 nm, setpoint control = 500 nN, relative setpoint = 2500 nN, z-closed loop active, z-length = 1 μm, retract delay = 10 ms, grid size = 25 × 25 μm², data point 35 × 35 per run with an orthogonal and lateral inter-sampling point distance of at least 715 nm. Low density polyethylene (LDPE) Riblene® FL30 foil was used as reference.