Table 1.
Intensity of callus meristem growth expressed as growth index (GI; see text) in barley single and double mutants with and without DMSO or HC toxin
| Mutant/hybrid | Control (H2O) | DMSO | DMSO + HC | Height of intact plant (cm) |
|---|---|---|---|---|
| WT (‘Auksiniai II’) (−) | 4·18 ± 0·57 | 3·18 ± 0·50 | 4·26 ± 0·66 | 75·2 ± 1·2 |
| Hv-tw (−) | 0·35 ± 0·08c3 | 0·70 ± 0·16c3 | 1·30 ± 0·24c3 | 71·7 ± 0·9 |
| Hv-tw2 (common mother) (−) | 3·90 ± 0·58 | 3·97 ± 0·54 | 2·82 ± 0·30a | 66·4 ± 1·33 |
| Hv-Lemma hooded (Lh) (−) | 1·46 ± 0·24c3 | 1·91 ± 0·35a2 | 1·61 ± 0·30c2 | 50·3 ± 1·03 |
| Hv-tw2 × Hv-Lh N13 (−) | 2·00 ± 0·33c2 | 1·84 ± 0·32a3 | 1·30 ± 0·22c3 | 49·3 ± 0·8 |
| Hv-tw2 × Hv-Lh N17 (+−) | 2·61 ± 0·52a | 2·08 ± 0·342 | 1·48 ± 0·19c3 | 57·9 ± 1·03 |
| Hv-tw2 × Hv-Lh N11-18 (+) | 2·17 ± 0·40b1 | 2·41 ± 0·222 | 2·86 ± 0·35 | 67·4 ± 1·33 |
| Hv-tw2 × Hv-Lh N19 (+) | 1·11 ± 0·18c3 | 1·65 ± 0·27b3 | 2·39 ± 0·48a | 70·6 ± 1·33 |
| Hv-Hooded (H) (−) | 2·03 ± 0·28c2 | 2·17 ± 0·252 | 2·00 ± 0·31b | 71·4 ± 1·2 |
| Hv-tw2 × Hv-H N8-20 (−) | 2·14 ± 0·25b2 | 1·51 ± 0·18b3 | 1·63 ± 0·19c3 | 75·2 ± 0·8 |
| Hv-Dense wing hood (Hv-Dwh) (−)* | 2·08 ± 0·42b1 | 2·35 ± 0·262 | 3·85 ± 0·391 | 30·4 ± 0·83 |
| Hv-tw2 × Hv-Dwh N25 (+) | 2·38 ± 0·30b1 | 2·55 ± 0·331 | 3·15 ± 0·50 | 80·8 ± 1·03 |
| Hv-Hoods on center spikelet (Hv-Hcs) (−)* | 1·59 ± 0·28c3 | 0·68 ± 0·11c3 | 2·82 ± 0·29a | 91·8 ± 1·13 |
| Hv-H × Hv-Hcs N9 (+) | 2·10 ± 0·20c2 | 2·07 ± 0·252 | 3·57 ± 0·45 | 89·8 ± 1·1 |
| Hv-Multiflorous (Hv-Mf) (−)* | 2·01 ± 0·51b1 | 2·23 ± 0·77 | 1·41 ± 0·38c2 | 44·2 ± 0·93 |
| Hv-tw2 × Hv-Mf N14 (+) | 2·45 ± 0·25b1 | 2·13 ± 0·252 | 2·77 ± 0·79 | 66·6 ± 0·93 |
*Accession numbers in USDA-ARS National Small Grains Collection are GSHO 928, GSHO 666 and GSHO 79, respectively.
(+,−) formation of leafy/shoot-like structures: (+), present, (−), absent; (+−), present under field conditions, absent in greenhouse.
a,b,cIn comparison with WT (wt) (‘Auksiniai II’): aP < 0·05; bP < 0·01; cP < 0·001.
1,2,3In comparison with the mother plant Hv-tw2: 1P < 0·05; 2P < 0·01; 3P < 0·001; similarly for plant height in the comparison of the corresponding mutant with WT (wt) or of father mutant with the corresponding hybrid.